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recombinant proteins pbs w o calcium  (Thermo Fisher)


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    Thermo Fisher recombinant proteins pbs w o calcium
    Recombinant Proteins Pbs W O Calcium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphate+buffered+saline+w+o/Phosphate-buffered+saline+(DPBS%2C+1X)%2C+Dulbecco's+formula%2C+without+calcium%2C+without+magnesium/pm42166331-55-8-14
    Average 99 stars, based on 1 article reviews
    recombinant proteins pbs w o calcium - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Infection:

    Article Title: Quantitative analysis of HSV gene expression during lytic infection
    Article Snippet: .. Materials Phase Lock Gel Heavy 2mL Tubes (5 PRIME, Catalog #2302830) HSV-1 infected cells/tissue of interest Dulbecco’s Phosphate Buffered Saline w/o Mg 2+ or Ca 2+ (DPBS) TRIzol (Life Technologies, Catalog #15596-026) BioMasher II Closed System Disposable Homogenizer (Kimble Chase, Catalog #749625-0030) Chloroform (Sigma Aldrich, Catalog #496189) Nuclease-free 1.5mL tubes Isopropanol 70% ethanol Nuclease-free water Note: The use of this protocol with phase lock tubes does not allow for recovery of DNA or protein and should be used exclusively for RNA extraction. ..

    Article Title: Quantitative analysis of HSV gene expression during lytic infection
    Article Snippet: .. Phase Lock Gel Heavy 2mL Tubes (5 PRIME, Catalog #2302830) HSV-1 infected cells/tissue of interest Dulbecco’s Phosphate Buffered Saline w/o Mg 2+ or Ca 2+ (DPBS) TRIzol (Life Technologies, Catalog #15596-026) BioMasher II Closed System Disposable Homogenizer (Kimble Chase, Catalog #749625-0030) Chloroform (Sigma Aldrich, Catalog #496189) Nuclease-free 1.5mL tubes Isopropanol 70% ethanol Nuclease-free water Note: The use of this protocol with phase lock tubes does not allow for recovery of DNA or protein and should be used exclusively for RNA extraction. ..

    Article Title: Quantitative analysis of HSV gene expression during lytic infection
    Article Snippet: .. Materials Cell of interest Small molecule inhibitor and vehicle control (optional) Dulbecco’s Phosphate Buffered Saline w/o Mg 2+ or Ca 2+ (DPBS) 0.25% trypsin-EDTA, phenol red (Life Technologies, Catalog #25200-056) DMEM/10%FBS (see Recipe) 37°C Incubator equipped with rocking platform Infection media (see Recipe) HSV-1 virus stock [see UNIT 14E.6 ( Arbuckle et al., 2014 ) for more information] Remove media from the extra well plated for counting cells. ..

    Saline:

    Article Title: Quantitative analysis of HSV gene expression during lytic infection
    Article Snippet: .. Materials Phase Lock Gel Heavy 2mL Tubes (5 PRIME, Catalog #2302830) HSV-1 infected cells/tissue of interest Dulbecco’s Phosphate Buffered Saline w/o Mg 2+ or Ca 2+ (DPBS) TRIzol (Life Technologies, Catalog #15596-026) BioMasher II Closed System Disposable Homogenizer (Kimble Chase, Catalog #749625-0030) Chloroform (Sigma Aldrich, Catalog #496189) Nuclease-free 1.5mL tubes Isopropanol 70% ethanol Nuclease-free water Note: The use of this protocol with phase lock tubes does not allow for recovery of DNA or protein and should be used exclusively for RNA extraction. ..

    Article Title: Quantitative analysis of HSV gene expression during lytic infection
    Article Snippet: .. Phase Lock Gel Heavy 2mL Tubes (5 PRIME, Catalog #2302830) HSV-1 infected cells/tissue of interest Dulbecco’s Phosphate Buffered Saline w/o Mg 2+ or Ca 2+ (DPBS) TRIzol (Life Technologies, Catalog #15596-026) BioMasher II Closed System Disposable Homogenizer (Kimble Chase, Catalog #749625-0030) Chloroform (Sigma Aldrich, Catalog #496189) Nuclease-free 1.5mL tubes Isopropanol 70% ethanol Nuclease-free water Note: The use of this protocol with phase lock tubes does not allow for recovery of DNA or protein and should be used exclusively for RNA extraction. ..

    Article Title: CD4 + to CD8 + T cell imbalance drives poor Achilles tendon repair in patients
    Article Snippet: White blood cells were isolated from 200 μl of whole blood or all available volume of hematoma aspirate by incubation for 12 minutes with 4 ml of lysis buffer (1x Red Blood Lysis (RBL) Buffer, eBioscience). .. After centrifugation the resulting cell pellet was washed and resuspended in FACS buffer: phosphate buffered saline w/o Mg/Ca (PBS, Gibco) with 1% fetal calve serum (FCS, Biochrom/Sigma-Aldrich). ..

    Article Title: Quantitative analysis of HSV gene expression during lytic infection
    Article Snippet: .. Materials MRC-5 cells (ATCC CCL-171) Water bath at 37°C Sterile 15mL conical tubes DMEM/10% FBS (see Recipe) Tissue culture dishes/vented flasks Dulbecco’s Phosphate Buffered Saline w/o Mg 2+ or Ca 2+ (DPBS) 0.25% trypsin-EDTA, phenol red (Life Technologies, Catalog #25200-056) Cell counter or hemocytometer Trypan Blue (Optional) Thawing and Passage of MRC-5 cells 1) Thaw one vial of MRC-5 cells (from ATCC) by gently swirling the tube in a 37°C water bath until just thawed. ..

    Article Title: Quantitative analysis of HSV gene expression during lytic infection
    Article Snippet: .. Materials Cell of interest Small molecule inhibitor and vehicle control (optional) Dulbecco’s Phosphate Buffered Saline w/o Mg 2+ or Ca 2+ (DPBS) 0.25% trypsin-EDTA, phenol red (Life Technologies, Catalog #25200-056) DMEM/10%FBS (see Recipe) 37°C Incubator equipped with rocking platform Infection media (see Recipe) HSV-1 virus stock [see UNIT 14E.6 ( Arbuckle et al., 2014 ) for more information] Remove media from the extra well plated for counting cells. ..

    Article Title: CD4+ to CD8+ T-Cell Imbalance Drives Poor Achilles Tendon Repair in Patients
    Article Snippet: White blood cells were isolated from 200 μl of whole blood or all 888 available volume of hematoma aspirate by incubation for 12 minutes with 4 ml of lysis buffer 889 (1x Red Blood Lysis (RBL) Buffer, eBioscience). .. After centrifugation the resulting cell pellet 890 was washed and resuspended in FACS buffer: phosphate buffered saline w/o Mg/Ca (PBS, 891 Gibco) with 1% fetal calve serum (FCS, Biochrom/Sigma-Aldrich). ..

    RNA Extraction:

    Article Title: Quantitative analysis of HSV gene expression during lytic infection
    Article Snippet: .. Materials Phase Lock Gel Heavy 2mL Tubes (5 PRIME, Catalog #2302830) HSV-1 infected cells/tissue of interest Dulbecco’s Phosphate Buffered Saline w/o Mg 2+ or Ca 2+ (DPBS) TRIzol (Life Technologies, Catalog #15596-026) BioMasher II Closed System Disposable Homogenizer (Kimble Chase, Catalog #749625-0030) Chloroform (Sigma Aldrich, Catalog #496189) Nuclease-free 1.5mL tubes Isopropanol 70% ethanol Nuclease-free water Note: The use of this protocol with phase lock tubes does not allow for recovery of DNA or protein and should be used exclusively for RNA extraction. ..

    Article Title: Quantitative analysis of HSV gene expression during lytic infection
    Article Snippet: .. Phase Lock Gel Heavy 2mL Tubes (5 PRIME, Catalog #2302830) HSV-1 infected cells/tissue of interest Dulbecco’s Phosphate Buffered Saline w/o Mg 2+ or Ca 2+ (DPBS) TRIzol (Life Technologies, Catalog #15596-026) BioMasher II Closed System Disposable Homogenizer (Kimble Chase, Catalog #749625-0030) Chloroform (Sigma Aldrich, Catalog #496189) Nuclease-free 1.5mL tubes Isopropanol 70% ethanol Nuclease-free water Note: The use of this protocol with phase lock tubes does not allow for recovery of DNA or protein and should be used exclusively for RNA extraction. ..

    Centrifugation:

    Article Title: CD4 + to CD8 + T cell imbalance drives poor Achilles tendon repair in patients
    Article Snippet: White blood cells were isolated from 200 μl of whole blood or all available volume of hematoma aspirate by incubation for 12 minutes with 4 ml of lysis buffer (1x Red Blood Lysis (RBL) Buffer, eBioscience). .. After centrifugation the resulting cell pellet was washed and resuspended in FACS buffer: phosphate buffered saline w/o Mg/Ca (PBS, Gibco) with 1% fetal calve serum (FCS, Biochrom/Sigma-Aldrich). ..

    Article Title: CD4+ to CD8+ T-Cell Imbalance Drives Poor Achilles Tendon Repair in Patients
    Article Snippet: White blood cells were isolated from 200 μl of whole blood or all 888 available volume of hematoma aspirate by incubation for 12 minutes with 4 ml of lysis buffer 889 (1x Red Blood Lysis (RBL) Buffer, eBioscience). .. After centrifugation the resulting cell pellet 890 was washed and resuspended in FACS buffer: phosphate buffered saline w/o Mg/Ca (PBS, 891 Gibco) with 1% fetal calve serum (FCS, Biochrom/Sigma-Aldrich). ..

    FACS:

    Article Title: CD4 + to CD8 + T cell imbalance drives poor Achilles tendon repair in patients
    Article Snippet: White blood cells were isolated from 200 μl of whole blood or all available volume of hematoma aspirate by incubation for 12 minutes with 4 ml of lysis buffer (1x Red Blood Lysis (RBL) Buffer, eBioscience). .. After centrifugation the resulting cell pellet was washed and resuspended in FACS buffer: phosphate buffered saline w/o Mg/Ca (PBS, Gibco) with 1% fetal calve serum (FCS, Biochrom/Sigma-Aldrich). ..

    Article Title: CD4+ to CD8+ T-Cell Imbalance Drives Poor Achilles Tendon Repair in Patients
    Article Snippet: White blood cells were isolated from 200 μl of whole blood or all 888 available volume of hematoma aspirate by incubation for 12 minutes with 4 ml of lysis buffer 889 (1x Red Blood Lysis (RBL) Buffer, eBioscience). .. After centrifugation the resulting cell pellet 890 was washed and resuspended in FACS buffer: phosphate buffered saline w/o Mg/Ca (PBS, 891 Gibco) with 1% fetal calve serum (FCS, Biochrom/Sigma-Aldrich). ..

    Sterility:

    Article Title: Quantitative analysis of HSV gene expression during lytic infection
    Article Snippet: .. Materials MRC-5 cells (ATCC CCL-171) Water bath at 37°C Sterile 15mL conical tubes DMEM/10% FBS (see Recipe) Tissue culture dishes/vented flasks Dulbecco’s Phosphate Buffered Saline w/o Mg 2+ or Ca 2+ (DPBS) 0.25% trypsin-EDTA, phenol red (Life Technologies, Catalog #25200-056) Cell counter or hemocytometer Trypan Blue (Optional) Thawing and Passage of MRC-5 cells 1) Thaw one vial of MRC-5 cells (from ATCC) by gently swirling the tube in a 37°C water bath until just thawed. ..

    Control:

    Article Title: Quantitative analysis of HSV gene expression during lytic infection
    Article Snippet: .. Materials Cell of interest Small molecule inhibitor and vehicle control (optional) Dulbecco’s Phosphate Buffered Saline w/o Mg 2+ or Ca 2+ (DPBS) 0.25% trypsin-EDTA, phenol red (Life Technologies, Catalog #25200-056) DMEM/10%FBS (see Recipe) 37°C Incubator equipped with rocking platform Infection media (see Recipe) HSV-1 virus stock [see UNIT 14E.6 ( Arbuckle et al., 2014 ) for more information] Remove media from the extra well plated for counting cells. ..

    Virus:

    Article Title: Quantitative analysis of HSV gene expression during lytic infection
    Article Snippet: .. Materials Cell of interest Small molecule inhibitor and vehicle control (optional) Dulbecco’s Phosphate Buffered Saline w/o Mg 2+ or Ca 2+ (DPBS) 0.25% trypsin-EDTA, phenol red (Life Technologies, Catalog #25200-056) DMEM/10%FBS (see Recipe) 37°C Incubator equipped with rocking platform Infection media (see Recipe) HSV-1 virus stock [see UNIT 14E.6 ( Arbuckle et al., 2014 ) for more information] Remove media from the extra well plated for counting cells. ..



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